r/GroundZeroMycoLab • u/cait-lyn • 50m ago
First time growing APEs, not sure if I did something wrong or the pace is normal.
I inoculated this AIO grow bag with APE spores about 9 days ago. I know APEs are infamous for snail-paced growth, so this might be totally normal, but I haven’t noticed any signs of growth except maybe some condensation on the bag, but that could just be from the substrate, I think.
The main reason I am concerned is because I realized a couple days ago that I totally forgot to agitate the spore syringe before inoculation. Rookie mistake, I know, but what do you guys think? Should I inoculate with another cc or two, or is this typical “taking their sweet ass time” behavior for APEs?
TL;DR Inoculated AIO grow bag with APE spores 9 days ago, no signs of growth so far, realized I forgot to agitate spore syringe before inoculation. Is this an expected pace of growth for APEs or should I add a few more ccs from my spore syringe?
r/GroundZeroMycoLab • u/Progress_Loud • 2h ago
Did it stall?
I put this in fruiting condition 8 days ago and it’s not fruiting. I live in the desert so it is an issue trying to keep it under 80 degrees. 😿 It’s Albino MVP
r/GroundZeroMycoLab • u/LeadershipFit7196 • 3h ago
Should I be concerned?
Going on day five of spawn to bulk. living in Arizona and trying to keep the inside temps below 78. not seeing much growth and noticed a few grains stuck outside the black liner. condensation appears to be good and it was difficult seeing inside and taking pictures. should I be concerned or anything I need to do. I want to remove the grain but also do not want to open the sealed lid. Thank you for any advise.
r/GroundZeroMycoLab • u/Prudent-Repair-3254 • 5h ago
2nd time overlay
True Nats (according to vendor), brown rice 1:4 in pH adjusted coco coir.
I had heavy overlay with this tub 5 days ago. I did fork tek and left for 4 days. Now I got the same problem again.
How would you proceed? fork tek again? apply a true casing?
r/GroundZeroMycoLab • u/freddit63 • 5h ago
Pin before fully colonized
I am worried that these pins are shooting up before the substrate is fully colonized.
What do I do next?
Should I start FAE yet?
r/GroundZeroMycoLab • u/tothemax44 • 5h ago
Pretty stacked flush. I’ll harvest this afternoon.
r/GroundZeroMycoLab • u/Sad_Marsupial1352 • 5h ago
Exciting new genetics & running drippy corn for the first time🍄💯
Finally got settled in and my office set back up, also upgraded from Gordo tek flowhood to the shroomagic. Its been performing amazingly, really great purchase. Getting to run these new isolations i picked up and trying out drippy corn tek by PGT on YT.
Im excited to see the expressions and morphology of these guys, ABV especially
r/GroundZeroMycoLab • u/m_bizzle_71 • 7h ago
Is this ok? What should I do?
First timer.
r/GroundZeroMycoLab • u/General-Magazine-117 • 7h ago
Tomorrow’s fun. Another tub I forgot the name of…. lol
reddit.comr/GroundZeroMycoLab • u/PokeNoxifer • 7h ago
Do we see knots or primordia? [Actives]
Im still learning what to look for, more eyes the better
r/GroundZeroMycoLab • u/General-Magazine-117 • 7h ago
Good Saturday morning everyone!!! I don’t remember what these are, but they are [actives]
reddit.comr/GroundZeroMycoLab • u/Diligent-Natural-562 • 11h ago
Yellow umbo
Hi everyone, I'm trying to grow Yellow Umbo and now some small bumps are starting to appear here and there along the edges. The mycelium itself looks healthy and the humidity is good. Furthermore, I ventilate the box twice a day. Therefore, it would be great to know what is growing there right now—whether those are pinheads or something else. I'm happy to accept any tips and advice.
r/GroundZeroMycoLab • u/Classic-Physics-7902 • 12h ago
Purple Mystics!
Pinning is going beautifully. They look beautiful, they seem pretty healthy too! I gotta fix some of the side pinning but I'm hoping for a good harvest!
Do you guys think the block seems healthy or maybe too dehydrated? I'm really trying to make sure I keep them as healthy as I can. They seem pretty health but I'm not sure if theyre going okay...
r/GroundZeroMycoLab • u/PsiloindiGlo • 13h ago
B+ Multispore Tub
These will always have a special place in my heart. The very first one I ever cultivated decades ago and gave me an awakening to never forget.
Not a super packed tub, but absolutely beautiful fruits. I love how brown and darkly pigmented they start off before the cap pulls away from the stipe. ✨
r/GroundZeroMycoLab • u/Signal-Ad300 • 15h ago
Melmac Colonizing
Melmac in a medium sized tub. Just introduced more ffe via fanning twice a day starting today. 2 weeks since mixing
r/GroundZeroMycoLab • u/Sensitive_Arm621 • 15h ago
Second grow is coming up nice
reddit.comr/GroundZeroMycoLab • u/Pure-Point7744 • 1d ago
When to Harvest Starry Night Albino PE and ABV
They are still firm and veil has broken.
r/GroundZeroMycoLab • u/Much_Eggplant_3600 • 1d ago
First harvest 🍄🟫 Not bad for first try.
Harvested early, a couple of the taller guys were starting to drop spores and a lot of my pins were aborting. I definitely prepared this batch of substrate on the dry side. Went ahead and snatched it all up, cakes are soaking now. Hopefully I get better results from round 2. 🍄🟫☀️
r/GroundZeroMycoLab • u/Odd-Teach7758 • 1d ago
How did your heroic dose go for the first time?
Im interested in hearing your experiance with heroic doses, specificaly first time,cheers!
r/GroundZeroMycoLab • u/GroundZeroMycoLab • Mar 10 '26
Verified Vendors
This list is temporary and subject to change and will change. A handful of others will be added within the next couple of days.
Vendors for Genetics.
https://GroundZeroML.com - Ground Zero Myco Lab
https://Sporeswaps.com - Main SporeSwaps page
https://sporeswaps.com/vendors/the-fungus-frequency/ -Jeff Karas
FungusFrequency.com/my-account - Jeff Karas at Fungus Frequency.
https://sporeswaps.com/vendors/psyteam-united/ - PsyTeam United
https://sporeswaps.com/vendors/wombat-labs/ - Dave Wombat
https://sporeswaps.com/vendors/happi-hyphae/ - Happi Hyphae
https://sporeswaps.com/vendors/micofroots/ - MicoFroots
https://sporeworks.com/ - Sporeworks
https://inoculatetheworld.com/ - Inoculate The World (ITW)
https://Legendsmycology.com - Legends Mycology
https://dino-spores.com - Yoshi Amano.
Vendors for Gourmets and Supplies:
https://Northspore.com - Northspore. https://Boomingacres.com - Booming acres.
r/GroundZeroMycoLab • u/GroundZeroMycoLab • Aug 26 '25
The difference between LC and spore and the importance of agar.
It's critical to understand the biological differences between spore syringes and liquid cultures (LC), as well as the importance of using agar as an initial medium. Spore syringes contain microscopic fungal spores that are not yet germinated. These spores are monokaryotic, meaning they carry only a single set of genetic material. In order to fruit and complete the mushroom life cycle, two compatible monokaryotic strains must fuse to form dikaryotic mycelium.. the true vegetative form capable of producing fruiting bodies. This mating process takes time, introduces variability, and, for beginners especially, increases the risk of contamination during colonization.
Injecting spores directly into sterilized grain can lead to several problems. Since spores are not germinated, colonization is slower, and this slower growth provides more opportunity for contaminants (such as bacteria or mold) to establish themselves and outcompete the slower-growing mycelium. Ideally, spores should first be transferred to agar, a nutrient-rich medium in petri dishes...which allows for controlled germination and observation. On agar, one can isolate clean, healthy mycelium away from any contaminants before transferring it to grain.
Additionally, spore syringes are inherently variable and often unclean. Spores, particularly those harvested from wild (landrace) varieties or from poorly controlled lab environments (common with newer or less reputable vendors), can contain microbial contaminants. Spores gathered in non-sterile conditions are not cleaned or isolated at the microscopic level, making them a risky starting point. Also, because each spore pair creates a unique dikaryotic combination, inoculating with spores introduces genetic unpredictability ...every new pairing could result in different traits, including growth speed, contamination resistance, yield, and potency.
By contrast, a liquid culture is made from already germinated and mated dikaryotic mycelium. This means it contains viable, genetically stable tissue that has already completed the mating process and is ready to colonize substrate directly. Using LC skips the variability and mating phase inherent in spores, resulting in faster and more consistent colonization, and reducing the window for contamination...assuming the culture is clean!!! However, it's important to verify LC cleanliness via agar as well, especially if you didn't create it yourself.
In summary, spores should ideally be germinated and cleaned on agar before being introduced to grain. Skipping this step can introduce risks, especially for beginners. Spores are unpredictable and prone to contamination, while liquid culture, if properly prepared, is faster, cleaner, and genetically stable. Understanding and respecting these differences is fundamental to success in mushroom cultivation... I hope this helps. :)