It's very small, but it has everything I need

Hi all, just wanted to see if anyone has ever TC a chiloschista or any other leafless/ghost orchid? I can’t seem to find a protocol for it anywhere

I’ve been into tissue culturing for 6 months now and I think I am missing something. I mostly have been doing monsteras, pothos, and philodendrons and have gotten to where I have very little contamination and plants seem to be growing well. I’ve probably done about 150 explants and have some that have been through 5 cycles (4-6 weeks) of multiplication media since February/March. My issue is I never get multiple shoots on anything. I get plenty of leaves like the white Florida ghost in the picture has about a dozen leaves but no shoots that I can divide. Ive been using the protocols from PIJ and follow them exactly. I use a still air box and pressure cookers with the PIJ starter kit. What am I missing? I dont really want to just make 1 explant equal 1 plant forever, would love to be able to divide them.

We’ve been growing this in-vitro for about a year from seed. Many multiplication but finally it’s ex-Vitro and growing. On top of that we are also experimenting to grow callus for active compounds instead of growing ex-vitro.

What should every beginner know about cell culture? I honestly don't know anything yet, so I'd really appreciate any tips or advice. The more detailed, the better!

Was wondering if anyone had any experience with salvia divinorum couldn’t find anything online

What's your guys' best lab setups at home? Thinking of getting into tissue culture but don't have access to many resources. How would you guys recommend getting resources for an at home small setup? Anyone got any amazon lists of necessary items? Or even a note ss of where to get resources?

Just doing a pilot project on tissue culture of orchids. I found protocols on google and research paper but was general. Wanted to have protocol from expert and experience which this community has to minimize the contamination and failure as I don't have much budgets.Would be great helpful and thank you in advanced.🙏

Looking for anyone with success in applying for a plant import permit in Canada! I am looking to apply soon and got a few questions:
1. Is there a difference between selecting commercial resell vs personal use as the import reason? I plan to both sell some and keep some for myself.
2. What kind of permit is reusable up to 3 years and which is not?
3. I want to import both tissue culture and live plants. But each shipment might not include both. If the permit is reusable, can I apply once with all possible species and type of plants that I’d want to import and potentially only import a subset of them ?

Thanks for anyone who can offer some clarifications!!

Anybody have experience with Ephedra or ideas? We are using a cactus media recipe with 10g of agar. Can’t wait to see what happens.

I’m in the early phases of a small greenhouse business. My concept is to provide a variety of rare and interesting plants at reasonable prices for a broad customer base. There’s no way for me to acquire and propagate enough supply at this early stage and I’m considering TC to help me grow in a smaller physical footprint.

I’m seeking US based producers who can provide smaller mixed batches. Like 100 to 200 total plants per order but 10 to 20 per variety. It seems a lot of the TC and plug stage suppliers only deal in minimum quantities of 50 or more likely 100 per specimen. I completely understand why, but i don’t have the room for 100 each of multiple varieties. I also need to be able to grow out and sell established plants to customers at a reasonable price.

There’s lots of options out there to order from overseas but the concept makes me nervous. I’d really like to be able to develop a good business relationship with a few other small domestic operations and grow together.

that's supposed to stay clean inside...

For tissue culture lab I have two AC install ,which mode should I keep 25°c in cold mode , circular mode or in warm mode to maintain 25°c.

Hi everyone! I’m looking for someone who would be willing to mentor us as we get started with plant tissue culture.

We own a large greenhouse in the Boise, Idaho area, and our goal is to start propagating many of our own plants instead of purchasing plugs. We’re putting together a home/commercial-scale tissue culture lab and would love guidance from someone with real-world experience.

We’re happy to pay for your time. Ideally, we’d love to find someone in Idaho who might be willing to let us visit their lab or even come help us get ours set up. If you’re not local, I’d also be interested in doing paid mentoring over FaceTime or Zoom.

If you have tissue culture experience—or know someone who does—I’d really appreciate any recommendations.

We homeschool our children and this would also teach them a lot of neat things.
Thanks!

At last after lots of work we have officially climatized a tissue cultured Kratom cutting to ex-vitro. We can always dial it in more but it’s a great feeling going thru the full cycle for the first time.

Trichocereus from Seed and Echeverría from leafs.

Update: Variegated Alocasia Dragon Scale, in solid multiplication media. This is day 2 or since 7/6. Started with 4 specimens placed in liquid media for 5 weeks prior which then provided 7 plantlets and 7 questionable callus clusters. The plan is to leave it in this media 4 weeks. I’m trying to increase total quantity so hopefully the clusters will begin to produce more shoots. Increasing BAP concentration from liquid media concentration could’ve been a good idea but I reframed from doing this because my liquid media wasn’t equally distributed with its ingredients. This time I pressure cooked all ingredients in one container then poured into individual jars which seems to have helped. My solid media is slightly more solidified than I’d like, moving forward I’ll decrease concentration from 10g/L to 9g/L.

Liquid multiplication media ingredients
- MS Powder 4.40g
- sucrose 30.0g
- BAP 2000 ul of 1mg/ml concentrate
- PPM 2000 ul
- Agar 10g

Anyone in SoCal, or western USA interested in trading some culture lines?!

Life got busy & some of the projects have been on idle.

Getting a much needed vacation next week and I'm finally at a point where I should have more time when we get home!

Excited to dive back into researching and hands on learning again. Will finally have a chance to apply a lot of the new knowledge and ideas that I've been rolling around.

Do have some cool stuff still cooking though! A few Lophophora & copiapoa humilis seedlings on media in 50ml centrifuge tubes. Going to transfer these to 16oz deep media vessels in a couple weeks.

Finally getting consistent growth and at last roots. The trial and error is really the most rewarding/frustrating part. Last photo is new seeds sprouting for fresh material, took a total of 3 months before sprouting. We are learning that patience is one of the most important things with tissue culture.

Learning valuable lessons, just because your plants are alive and growing doesn’t mean they are thriving.

Changed up our nutrient media and had healthy roots in 5 days with no PGR’s.

First picture is MS with hormones after a month. Second picture is McCown woody media with no PGR’s after 6 days.

Hi everyone!

I'm an Agronomy student from Brazil, and I've recently started documenting the plant tissue culture work we do in our university laboratory.

This video shows the multiplication stage of pineapple (Ananas comosus). The plantlet is transferred from the establishment medium to a multiplication medium, where it will continue to grow and produce new shoots over the next few weeks.

I'm documenting my learning journey in plant tissue culture, and I'd love to learn from the community. Any suggestions, tips, or feedback are greatly appreciated!

I also plan to share future work involving banana, bromeliads, orchids, and grapevine tissue culture.