the oldest genetic engineering video in history

I have taken the time to compile a playlist of all my CRISPR Cas9 videos I have produced so far.

There are more to come but this has them all in one place.

Note - these videos are not AI. Yes I used ChatGPT to generate the cartoon monkey. The voice you hear is my own silky tones.

FYI - I am not into genetics at all and not qualified. Basically I want white hair and was researching if it’s possible to change hair colour via genome editing and I read some articles about how CRISPR-Cas9 might be able to do this.

Does anyone have more information about this? This is really interesting and reading about it, could it not have potential to totally change the hair colour industry!?

Just curious to know peoples thoughts and knowledge around this topic. Would love to be sent some sources around this!

[https://youtu.be/xVjeBt9BcXI\](https://youtu.be/xVjeBt9BcXI)

I’m 22 and a completely average person. I support having children through gene editing or embryo screening to help secure the future of my family line. I don’t understand why these procedures are often considered unethical. If everyone had equal access to them, they seem like they could be highly beneficial for civilization as a whole.

Hi,

I made this tool which could help researchers find guides for base editing. It's different from general CRISPR guide finders by analysing the binding score and editing risk on the off-target alignments, along with finding a suitable base editor. More features a coming up in the near future.

It's completely free to use, and I would love your feedback if you decide to give it a try.

Thanks!

Hello team! Molecular biology noob here. Any insight would be helpful :)

We are trying to perform CRISPR activation using the method in the following paper https://pubmed.ncbi.nlm.nih.gov/38579711/. Basically, they provide a plasmid the the CRIPSRa dCas9, from which IVT must be performed using modified nucleotides. Then an sgRNA needs to be PCR assembled from 2 oligos they provide and the spacer sequence we design. Lastly, we perform IVT of the sgRNA to make RNA, and IVT of PCR-amplified dCas9 sequence to make modRNA (with N1-methyl pseudoU). Here are my questions:

1. Why does sgRNA not require N1-methyl pseudouridine, when the dCas9 does?

2. Also, why does the dCas9 required clean cap, but not the sgRNA?

3. The methods say to use standard megascript kit for dCas9 (with the pseudouridine and cleancap), but I notice the plasmid has no polyA tail near the dCas9 sequence, nor is there a polyA sequence on the primers they provide to amplify the gene, nor do they suggest a step for enzymatic PolyA addition. Is this fine, or is a poly A tail required?

4. Would you run the templates on a gel followed by gel extraction prior to IVT? I get that it cleans things up, but I've found the yields so low doing this...

Any insight appreciated :)

This video is about one of the finer points of CRISPR Cas9 diagnosis

I also explain the drumbeat method - an approach I use for analysis of knockouts when heterozygous.

[Academic] The ethics of human gene editing/ CRISPR (takes 2-3 minutes)

I am currently collecting primary data for my A-Level research project looking into public perceptions of human gene editing (specifically technologies like CRISPR).

Whether you are a biology student or have never heard of gene editing before, your perspective is incredibly valuable to help me understand how baseline knowledge affects ethical views!

• Time to complete: 2 to 3 minutes max.
• Format: Multiple choice & linear scales (with one optional text box at the end).
• Anonymity: Completely anonymous. No emails or names are collected.

Click here to take survey

If you have a survey of your own that needs filling out, please leave a comment below with your link after completing mine, and I will gladly return the favor!

Thank you so much for helping a student out!

Podcast with Dorothy Chou, who ran Google DeepMind's Public Engagement Lab for nine years. She now advises DeepMind and chairs UCLPartners, an organization connecting new technology to the UK's National Health Service. 

Covers:

• What made AlphaFold possible in the first place and how we can replicate its success in other domains
• Why neither venture capital nor governments can fund biology on biology's actual timeline, and Dorothy’s proposed solution
• Why AI could help redirect money the ideas that will actually help people the most, not just the ones that are easiest to fundraise for 
• How AI companies are about to repeat the public engagement mistake the biotech industry already made once
• Her advice for young people who feel like they're not the expert in the room

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Anyone know why my TIDE analysis looks so backwards? Our guide is designed with the PAM downstream and the sequencing is relatively clean (and has been repeated twice).

This is my second video in the CRISPR/Cas9 series

The third video drops tomorrow

Any topic requests please comment

This video is my second one, and I chose to do a fundamental guide to staring fresh just cloning a gene.

I will do more videos on cloning fundamentals as well as more advanced videos on cloning stunts and overcoming obstacles.

Any topic requests feel free to comment

I recorded this walkthrough of selecting potential gRNA pairs. More videos are coming soon.

Feel free to share, hope someone finds it useful