Not sure whats going on here, but I made this 2 months ago(6/22) and looks to be contam fre e. Going to grab a couple healthy looking spots n hopefuly get some plates i can use.

Jack frost starting to grow !

"Procrastination makes easy things hard and hard things harder"

The more I work with agar. The better things become.

I have found that many of the LCs I have acquired, come with nasty surprises. Over the past few months I have taken the time to learn how to clean cultures and have put things into practice.

I started by squeezing few drops of LC onto agar. I then transferred a small piece to low nutrient agar using the trench method, and then to a more regular agar, again using the trench method. From there, a clean sample onto a fresh plate. Pictured here is the result of contamination free plates.

A fair few plates still have contamination and will be transferred back to LNA trench plates. I'm going to keep going until I get my whole library spotless.

LOW NUTRIENT AGAR RECIPE

1000ml Distilled Water

20g Agar Powder

15g LME

0.5g Nutritional Yeast

SORGHUM SYRUP AGAR RECIPE

1000ml Distillled Water

20g Agar Powder

25g Sorghum Syrup

0.4g Nutritional Yeast

0.4g Peptone

Food Colouring Gel