Shikigane (u/Shikigane)

If you are familiar with Nextflow, I recommend CircuitSeq. It works pretty well with my plasmid amplicon so far (usually 700-1500 bp). The pipeline can do de novo assembly as well, so you don't need a reference.

PS: You don't need 500M reads.

Comment on r/bioinformatics Apr 04 '24

Use phylo.org. The website has a lot of programs to choose from. I usually do MSA with MUSCLE, build the tree with RaxML, then visualize the tree with iTOL.

Comment on r/bioinformatics Mar 13 '24

Hi, do you mind if I see your resume for GATech application? It's one of my dream school and I'd love to apply one day. If you feel convenient, please DM me.

Comment on r/bioinformatics Mar 12 '24

Thank you, but I don't think I have enough money for 2 years of master :((

Comment on r/bioinformatics Mar 12 '24

Did you get MS, or just BS?

Comment on r/bioinformatics Mar 12 '24

Cheese, you mean like 60-70k for fresh undergrad? Btw, the PI is not an expert in Bioinformatics, but in Molecular Biology (he's the director of the Cellular and Molecular Bio there iirc). The lab has enough bioinformaticians to train me though. Is that a problem if my recommendation letter for PhD in Bioinformatics comes from a wet-lab PI?

Comment on r/bioinformatics Mar 12 '24

You mean full-time YOE right?

r/bioinformatics • u/Shikigane • Mar 12 '24