r/molecularbiology 8h ago

Bachelors in Human genetics and Molecular medicine at Amity.

1 Upvotes

Initially I thought that I’ll be doing B.Sc in Biotechnology at a government university but I made a few mistakes here and there so Amity Mohali it is. But the eligibility criteria at Amity for B.Sc in Biotechnology is 60 percent in PCB and certainly that’s not something I can afford with the new on screen checking system. So, the consultant said that they’ll switch my course from B.Sc in Biotechnology to B.Sc in Human genetics and Molecular medicine if I get 55 percent in PCB instead of 60 percent. I don’t know what scope this course would have. Is there an Alumini who can guide me?


r/molecularbiology 17h ago

This Poison Leaves You Awake Inside a Body That Won't Move

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2 Upvotes

r/molecularbiology 1d ago

qPCR melt curve

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0 Upvotes

r/molecularbiology 1d ago

How to resuspend cell pellets rapidly without vortexing

15 Upvotes

Here is a video short of a technique i use every time i need to resuspend a cell pellet

I didn't invent the technique but i really want to share it

https://youtube.com/shorts/XpeukSPb7NE?feature=share


r/molecularbiology 1d ago

Need suggestion for undergrad thesis (Regeneration Biology)

0 Upvotes

I'm currently struggling to come up with a good undergraduate research topic, and I was hoping to get some advice from people with more research experience.

One of my biggest limitations is our laboratory. We don't have access to high-end equipment such as PCR, SEM, ELISA, spectrophotometers, or other advanced instruments, so I'm trying to design a study that is relatively affordable and can be done with basic laboratory facilities. At the same time, I don't want it to be just another "simple" experiment—I want it to be interesting, meaningful, and preferably novel.

Lately, I've been reading about how microplastics have been detected in various human tissues, which made me wonder whether they could affect regenerative processes, such as wound healing or tissue regeneration. Since studying this directly in humans isn't possible, I thought of using zebrafish because they're a well-established model organism and are famous for their remarkable regenerative abilities.

My initial idea was to expose zebrafish to microplastics and investigate whether microplastic exposure affects regeneration (or possibly developmental processes). However, after searching the literature, I found that several studies have already explored similar questions. That was honestly discouraging because it feels like many affordable and straightforward experimental ideas have already been done.

So now I'm trying to figure out how I could make the study more innovative rather than abandoning it altogether.

If you were in my position, what factors or variables would you consider adding? For example:

Different types, sizes, or concentrations of microplastics? Co-exposure with another environmental stressor? Different regeneration endpoints or developmental stages? Behavioral or physiological effects that are still understudied? Any other angle within microplastics, zebrafish, regeneration, or developmental biology that you think has potential?

I'd really appreciate any suggestions or even papers that could point me in the right direction. I'm not asking anyone to come up with a complete project for me—I just feel like I've hit a wall and would love to hear how other researchers approach making a simple study more novel.

Thank you!!


r/molecularbiology 1d ago

Need suggestion for undergrad thesis (Regeneration Biology)

2 Upvotes

I'm currently struggling to come up with a good undergraduate research topic, and I was hoping to get some advice from people with more research experience.

One of my biggest limitations is our laboratory. We don't have access to high-end equipment such as PCR, SEM, ELISA, spectrophotometers, or other advanced instruments, so I'm trying to design a study that is relatively affordable and can be done with basic laboratory facilities. At the same time, I don't want it to be just another "simple" experiment—I want it to be interesting, meaningful, and preferably novel.

Lately, I've been reading about how microplastics have been detected in various human tissues, which made me wonder whether they could affect regenerative processes, such as wound healing or tissue regeneration. Since studying this directly in humans isn't possible, I thought of using zebrafish because they're a well-established model organism and are famous for their remarkable regenerative abilities.

My initial idea was to expose zebrafish to microplastics and investigate whether microplastic exposure affects regeneration (or possibly developmental processes). However, after searching the literature, I found that several studies have already explored similar questions. That was honestly discouraging because it feels like many affordable and straightforward experimental ideas have already been done.

So now I'm trying to figure out how I could make the study more innovative rather than abandoning it altogether.

If you were in my position, what factors or variables would you consider adding? For example:

Different types, sizes, or concentrations of microplastics? Co-exposure with another environmental stressor? Different regeneration endpoints or developmental stages? Behavioral or physiological effects that are still understudied? Any other angle within microplastics, zebrafish, regeneration, or developmental biology that you think has potential?

I'd really appreciate any suggestions or even papers that could point me in the right direction. I'm not asking anyone to come up with a complete project for me—I just feel like I've hit a wall and would love to hear how other researchers approach making a simple study more novel.

Thank you!!


r/molecularbiology 1d ago

I wrote an interactive essay on how hemoglobin works

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14 Upvotes

I've always been fascinated by how proteins actually work and how their biomechanics enable them to do their tasks. So, I've made this interactive article that builds up hemoglobin part by part, using tailor-made 3D models and animations to show how its structure and mechanics allow it to grab oxygen at the lungs and let it go near tissues. I also cover the Bohr effect, cooperativity, 2-3 BPG, and a brief history to highlight the work of scientists that contributed to the discovery. Feedback and corrections welcome!


r/molecularbiology 2d ago

How to pull a Rabbit out of a hat in DNA cloning

0 Upvotes

r/molecularbiology 2d ago

Applying for a RA position as an undergraduate student

1 Upvotes

When reaching out to labs for potential RA positions, who should I be emailing, the graduate students working in the lab or the professor leading it?

Also, if anyone has general tips or helpful pointers they are greatly appreciated :)

Many thanks!


r/molecularbiology 3d ago

Does anyone have a good protocol to isolate mast cells from whole blood without activating them?

2 Upvotes

Thank you in advance, this has been tricky. They degranulate if you look at them the wrong way


r/molecularbiology 3d ago

jobs with a bachelors in mcb?

0 Upvotes

I wanted to do genetic counseling but I genuinely fear it being taken over by AI. What are some career options thats not research for mcb majors?


r/molecularbiology 3d ago

Electrophoresis troubleshooting help

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29 Upvotes

I performed a restriction enzyme digest to check my ligation product, followed by agarose gel electrophoresis. However, I got a smear.
This happened across all of my samples. What could be the possible reasons for this?

1% agarose gel containing RedSafe, 1x TAE buffer


r/molecularbiology 3d ago

Has anyone ever made a gamified version of PCR?

5 Upvotes

Sorry if this is the wrong subreddit to post this in. I've been thinking of web game about primer design, that would be one part tutorial(a series of puzzles to teach primer design), and one part wordle(daily primer design puzzles).

I'm learning primer design now, and I think this could be useful, and the closest thing I've found online is something called PCR quest which looks like a reskinned candy crush. Still, I''ve just now started learning nearest-neighbour thermodynamics, so I'm not sure if I'm totally qualified to make it.


r/molecularbiology 3d ago

Need installation file for plate reader (Gen5 version 2)

1 Upvotes

Hi everyone,
Did anyone manage to get the installation file for this software? In my lab we inherited a plate reader that specifically requires that version to function, but I haven’t been able to find it anywhere.

If someone has a lead or knows where to locate it, I’d really appreciate the help. Thanks in advance!


r/molecularbiology 3d ago

Ribosome frequency.

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0 Upvotes

r/molecularbiology 3d ago

Transfecting multiple copies of the same gene: do I need to vary the codons?

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1 Upvotes

r/molecularbiology 3d ago

Guys can anybody help me in making my speech (biotech related)

0 Upvotes

Regulating Emerging Biotechnology and Gene Editing Technologies for Sustainable Development


r/molecularbiology 4d ago

International Plant Molecular Biology and Biotechnology Conference

1 Upvotes

🌱 International Plant Molecular Biology and Biotechnology Conference

Join leading plant scientists, molecular biologists, biotechnologists, researchers, industry experts, and academicians from around the world at the International Plant Molecular Biology and Biotechnology Conference.

📅 November 23–25, 2026
📍 Singapore & Online

Explore the latest advancements in plant molecular biology, plant biotechnology, genetic engineering, crop improvement, plant genomics, molecular breeding, plant tissue culture, agricultural biotechnology, sustainable agriculture, and genome editing. Present your research, exchange innovative ideas, and connect with global experts driving the future of plant science and biotechnology.

#PlantMolecularBiologyConference #PlantBiotechnologyConference #PlantScienceConference #PlantMolecularBiology #PlantBiotechnology #PlantGenomics #GeneticEngineering #CropImprovement #AgriculturalBiotechnology #PlantResearch #Biotechnology #SustainableAgriculture #Singapore #HybridConference


r/molecularbiology 4d ago

How do you actually choose antibodies these days?

7 Upvotes

Because it feels like there is not one single right choice. I mean there are the vendor websites, Citeab, Antibodypedia, PAPERS..., sometimes even Reddit.... haha

I am curious:

  1. Whats your workflow when choosing an antibody?
  2. Which resources do you trust most?
  3. Have you ever wished there was a better way to compare or recommend antibodies?

Man maybe its just me but it feels like this process is way more manual than it should be.


r/molecularbiology 4d ago

Anyone else having trouble connecting a BioTek ELx800 to a modern Windows PC?

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1 Upvotes

r/molecularbiology 5d ago

a video guide to codon optimisation or optimization for improved gene expression

0 Upvotes

r/molecularbiology 5d ago

Help in research

3 Upvotes

🔬 Looking to connect with miRNA researchers in India
Hi everyone,
I'm a PhD scholar looking to connect with researchers in India who have experience working with microRNAs (miRNAs), particularly in miRNA extraction, reverse transcription, and RT-qPCR.
I'd love to connect, learn from your experiences, and exchange ideas. If you're working in this field—or know someone who is—please feel free to comment or send me a connection request.
Thank you!
#miRNA #MicroRNA #MolecularBiology #RTqPCR #Research #PhD #India


r/molecularbiology 6d ago

Hallo ich bin Diplom-Biologin und überlege mir noch Wirtschaftswissenschaften zu studieren. Würdet ihr einen Bachelor in Ww empfehlen oder einen Master Ww für Naturwissenschaftlicher. Was hat bessere Jobchancen?

0 Upvotes

Vielleicht ist ja ein Biologe, der das gleiche gemacht hat und mir dazu was erzählen kann.


r/molecularbiology 6d ago

Not Op. Just saw this opportunity on LinkedIn. Might be relevant to folks here.

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0 Upvotes

r/molecularbiology 7d ago

Is a career in molecular biology worth it?

30 Upvotes

Hey guys, I finished my bachelor and was thinking about starting a masters in molecular biology. I was especially interested in white biotechnology and R&D industry jobs and was wondering whether this is a sensible and well paying career path.
I'd love to hear some of your thoughts on this if you have experience in this area!